When you want to characterize a gene or protein of interest, you must first study its function. In this molecular age, obtaining a cDNA of your gene of interest is not difficult.

To express the cDNA as a protein, IE. a recombinant protein, one can then easily perform functional studies using purified recombinant protein.

Once you have a purified protein can lead to:

* Experience interaction Protein-protein
* Kinetics of enzymes
* Functional Studies of the protein
* Structural Studies – including protein crystallization, the protein structure and NMR.
* You can also use protein to make antibodies for further experiments.

There are two main systems for the expression of the recombinant protein. Once you get your cDNA copied, you must decide where you want to boost your protein. This will (usually a prokaryotic (bacterial) or eukaryotic yeast or mammalian cells). Choosing your system decide what vehicle you need to copy your cDNA as there are various promoters that function in Escherichia coli and others that work well with yeast or mammalian systems.

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• The dihedral angle ? is defined by four successive atoms skeletal CO-NH-C?-CO, the first being the carbonyl of the preceding residue.
• The dihedral angle ? is defined by four successive backbone atoms NH-C?-CO-NH, the second being that of the amide residue following.

Ramachandran plot of a protein. The energetically favorable areas are represented by colored contours. Each amino acid is represented by a red dot. The crosses correspond to the amino acid glycine, which does not contain side chains. All values of angles ? and ? are not possible because some lead to too close contacts between atoms that are energetically unfavorable. A systematic study of combinations of allowable angles ? and ? has been made by the Indian physicist and biologist Gopalasamudram Narayana Ramachandran in 1963 [2]. He devised a graphical representation of the space (?, ?) that bears the name of Ramachandran diagram. This diagram shows three main areas energetically favorable. When analyzing a protein structure, we observe that most of the amino acids have combinations of angles (?, ?) that fall within these areas. The two main regions correspond to regular secondary structures are mainly observed in proteins: the ? helix region and the ? sheets. The third area is smaller, corresponding to a left-handed helix conformation (?> 0). There are two specific amino acids that are exceptions to this rule diagram Ramachandran: glycine and proline. Glycine has no side chain (R = H) and, thus, is much less constrained in terms of steric hindrance. It can adopt values (?, ?) much more diverse in regions outside the normally preferred. In contrast, proline is more constrained: it contains a pyrrole cycle that prevents the rotation corresponding to the angle ?.

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Proteins are macromolecules of the cell, they constitute the “toolbox”, allowing it to digest its food, its energy, manufacturing its constituents, move. They consist of a linear sequence of amino acids linked by peptide bonds. This sequence has a three-dimensional organization (or folding) of its own. Sequence in the folding.

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If you just only eat protein bags, you will not have the necessary fiber to your intestinal balance and may experience stomach problems. In fact, the ingested food will lose their water and become more compact, to cause a risk of blockage of the intestine.

Fiber needs water to do their job effectively and not cause too significant residual deposits in the liver and kidneys. They go hand in hand with a consumption of 1.5 to 2 liters of water per day.

During the starter phase, the main fiber will be made by the salad and vegetables, low Glycemic and high intake of insoluble fiber that you have the right to consume, and possibly nutritional supplements that your doctor or dietitian will be required. These insoluble fibers have the function to increase the volume and frequency of bowel movements, stimulating the digestive tract.

During the transition phase, you gradually reintroduce the natural soluble fiber contained in legumes and citrus fruits in particular. They promote the removal of food residues in feces.

During the stabilization phase, a balanced diet with a daily intake of fruits and vegetables, you will ensure an adequate intake of fiber.

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This system allows you to lose fat, but while retaining muscle. In fact, muscles need a lot of energy to operate, and this fuel is supplied to them by proteins. More muscle mass, the greater the amount of energy burned is important. In this way, proteins are fighting cellulite fast! Down with orange peel, and the bright peach!

Since the high protein diet severely restricts or removes fat intake and sugar, the body will use its own reserves (glycogen, liver and muscle) to convert them into amino acids, before tackling its fat stores. In so doing, the body produces  fatiacid, which have the advantage of being euphoric and appetite suppressants, but the disadvantage that smell bad (remember to refresh your breath more often than usual ).

Finally, meal replacements and high-protein bars give you all the proteins necessary for good health. However, an injection of vitamins and minerals is essential to prevent deficiencies.

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Within the wide range of food with which we have these are classified according to their main function. There are 3 major food groups, the Builders, the Energy and Misc. The success of a good food supply is to combine the three main food groups for each child (breakfast, lunch and dinner). Talk about them and their importance in young children.

They are called builders because they are like bricks that form the body, and these are the foods rich in protein.

PROTEINS
The proteins not only form the muscles, but are the raw material of all the hormones and enzymes in the body, and constitute the internal organs and immune systems, which are in full training during childhood. Forman all other structures such as blood, hair and skin. Allow cells to reproduce and grow. These foods are essential for children because it is the key step in the development and growth.

Foods representatives of this group are: meat (any animal alike, including muscle, liver, kidneys, bofe, etc..), Eggs (the white is composed almost entirely of high quality protein), milk and its derivatives, legumes (beans, lentils, peas, soybeans)

Food of animal origin (meat, eggs, milk) contain complete proteins. The vegetable, however, when combined with each other and combine them with other food groups that we will see later, can achieve complement one another, thereby overcoming its lack of some amino acids (components of proteins) essential.

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